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TRT with Free Testosterone Blood Test

TRT with Free Testosterone Blood Test


An advanced TRT hormone monitoring profile which includes free testosterone, oestradiol and prolactin.
Blood sample 6 biomarkers included Results estimated in 2 working days from sample receipt at lab
TRT with Free Testosterone Blood Test
TRT with Free Testosterone Blood Test
TRT with Free Testosterone Blood Test
TRT with Free Testosterone Blood Test

Is it for you?

Are you taking testosterone replacement therapy and want a thorough monitoring profile which includes free testosterone, prolactin and oestradiol? Are you experiencing symptoms of low testosterone even though your total testosterone level is normal? This monitoring profile with free testosterone has been developed with a specialist TRT doctor and is perfect for anyone who wants to monitor their hormones whilst taking TRT.

What's included?

Biomarker profiles

  • Hormones
  • Proteins

Hormones (4 Biomarkers)

Proteins (2 Biomarkers)

Special instructions

Prepare for your TRT with Free Testosterone Blood Test by following these instructions. Please take your sample before 10am. Hormonal contraception can affect the results of this test. Taking a break from this and waiting for your periods to restart before your blood test will give more accurate results. Avoid taking your sample from a finger used to apply hormone gels/pessaries/patches in the past 4 weeks. Use gloves to apply these. Do not take biotin supplements for two days before this test, discuss this with your doctor if it is prescribed. If you are a woman take this test two to five days after the start of your period, ideally on day three. It can be taken any time if you do not have periods.

From the expert

This test profile has been designed for men taking testosterone therapy who want to monitor their male and female hormones. This test includes total testosterone as well as a calculation of the amount of free or unbound testosterone that is available to your tissues. Most testosterone in the body is bound to proteins (sex hormone binding globulin and albumin) and is unavailable to your cells. By measuring your total testosterone and your levels of SHBG and albumin, the laboratory can calculate your level of free testosterone. If you have high levels of SHBG then your free testosterone will be lower, even when your total testosterone is normal. Low free testosterone can cause symptoms such as low libido, lack of muscle mass, and low mood. It is also important for anyone taking TRT to check the levels of female hormones in the blood as high levels of testosterone can also cause high oestradiol. This is especially important if you are experiencing symptoms which are associated with high oestradiol (including growth of breast tissue and low libido).

Monitoring your hormone levels allows you and your practitioner to optimise your dose of testosterone replacement.

Dr Sam Rodgers MBBS, MRCGP
Chief Medical Officer

Why take this test?

  • You are taking testosterone replacement and you want a detailed look at your male and female hormones
  • You are experiencing symptoms of low testosterone and want to check your level of free testosterone
  • You have changed your dose of testosterone and want to ensure that your hormone levels are optimal
  • You are developing symptoms such as increased breast tissue associated with high oestradiol and you want to check your levels

Laboratories you can trust

Trusted by the NHS and private clinics alike, you can be sure of the highest testing standards from our UKAS accredited partner laboratories.

Dr Sam Rodgers - Chief Medical Officer

Expert advice with your results

Get trusted advice and comments from one of our doctors based on your results.

Your personal health centre

Your personal health centre

Discover the easy way to track your health through our online portal, View your results with doctor's advice, monitor any changes over time and see the improvements you can make to your health with simple lifestyle changes.

Questions? Let us help
Questions? Let us help

Questions? Let us help

Our customer care team is on-hand to help you find the test that's right for you. Get in touch via phone, live chat or email.


Our tests are not a substitute for seeing your doctor, especially if you are suffering symptoms. Our doctors will interpret your results based on the information you have provided, but will not diagnose, consult or provide any treatment. You will be advised to see your doctor for any necessary follow-up action.