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Advanced TRT (Testosterone Replacement Therapy) Blood Test

Advanced TRT (Testosterone Replacement Therapy) Blood Test


A comprehensive health check for men taking, or considering, TRT (testosterone replacement therapy). Includes tests for hormones, liver, kidney, thyroid function and prostate.
Blood sample 43 biomarkers included Results estimated in 2 working days from sample receipt at lab
Advanced TRT (Testosterone Replacement Therapy) Blood Test
Advanced TRT (Testosterone Replacement Therapy) Blood Test
Advanced TRT (Testosterone Replacement Therapy) Blood Test
Advanced TRT (Testosterone Replacement Therapy) Blood Test

Is it for you?

Are you experiencing symptoms of low testosterone and wondering whether you'd benefit from testosterone replacement therapy? Do you want a thorough hormone MOT to establish your levels before you start? Do you want a baseline check of your liver, kidney and PSA levels so that you can monitor them during treatment? This gold-standard TRT check gives you everything you need to manage your testosterone replacement therapy.

What's included?

Biomarker profiles

  • Hormones
  • Proteins
  • Liver Health
  • Cholesterol Status
  • Kidney Health
  • Diabetes
  • Iron Status
  • Thyroid Hormones
  • Clotting Status
  • Red Blood Cells
  • White Blood Cells
  • Prostate
  • Muscle Health

Hormones (6 Biomarkers)

Proteins (4 Biomarkers)

Liver Health (3 Biomarkers)

Cholesterol Status (6 Biomarkers)

Kidney Health (2 Biomarkers)

Diabetes (1 Biomarker)

Iron Status (1 Biomarker)

Thyroid Hormones (3 Biomarkers)

Clotting Status (2 Biomarkers)

Red Blood Cells (7 Biomarkers)

White Blood Cells (6 Biomarkers)

Prostate (1 Biomarker)

Muscle Health (1 Biomarker)

Special instructions

Please take your sample in the morning. Avoid strenuous exercise for 48 hours prior to testing. Please avoid ejaculating for 48 hours prior to this test. If you are taking a T3 containing medication take your test in the hour prior to your dose of medication. Fasting is optional for this test. Eating fatty foods might affect the results of a small number of blood tests so we may advise a retest when you are fasted. If you are interested in your triglyceride or LDL levels, then we do recommend fasting. Do not take biotin supplements for 2 days prior to this test. If you are taking prescribed biotin you should discuss this with your doctor. Avoid taking a finger-prick sample from a finger used to handle hormone-gels in the past 4 weeks. Avoid heavy exercise and eating meat-rich meals for 48 hours before your test.

From the expert

The Advanced TRT (Testosterone Replacement Therapy) Blood Test is a venous blood test (which means it must be taken from a vein in your arm) which measures important markers in your blood to help you monitor and manage your testosterone replacement therapy. While testosterone can have a positive impact on strength, libido, sexual performance and mood, there can be risks to health, especially with over-supplementation. 

This check includes tests for red and white blood cells, liver and kidney function, cholesterol (high doses of testosterone can reduce HDL "good" cholesterol) and thyroid function. It includes a test for prostate specific antigen (PSA), a protein which can be raised in cases of prostate cancer (although it can be raised in benign conditions as well). Taking TRT does not appear to raise your risk of getting prostate cancer but having high levels of testosterone can make prostate cancer worse. This is why it is important to have a physical examination of your prostate gland before taking TRT. It is also important for men taking TRT to regularly check their PSA levels, but you should be aware that there are shortcomings to testing PSA which are detailed below. 

Elevated testosterone can impact levels of other hormones in your body. This profile includes tests for testosterone, free testosterone (calculated), oestradiol, prolactin and SHBG. We recommend taking this test before starting TRT and 6 months after for monitoring purposes.

You should understand that this test includes a check on your Prostate Specific Antigen (PSA) level. Whilst current research indicates that it is unlikely that testosterone replacement therapy increases the risk of prostate cancer, caution is still advised, particularly for men who already have prostate cancer. 

Whilst the PSA test can help to detect prostate cancer earlier it has several shortcomings when used as a screening test. Besides being increased by prostate cancer PSA can also be increased by innocent conditions such as urinary tract infection, recent ejaculation and even some forms of exercise.. This means that there is a risk of PSA being positive when no cancer is present (a false positive). If we take 100 men with a high PSA result then approximately 75 of them will have a false positive result, and 25 will have a true positive (i.e. they will have prostate cancer). Currently the most reliable way of working out whether someone has a true positive or a false positive is by performing a prostate biopsy. Because the PSA test has a high false positive rate many of these prostate biopsies will have been unnecessary.  

You should also be aware that PSA tests can cause false negative results. This means that the PSA is in the normal range but the person has underlying prostate cancer. PSA levels can remain normal in the early stages of prostate cancer, providing false reassurance. If we test 100 men with prostate cancer then approximately 15 of them would have a normal PSA result (a false negative). In some cases prostate cancer grows very slowly and may never cause symptoms or shorten lifespan, so a significant proportion of the men who receive a true positive result may undergo surgery that they did not need.

Dr Sam Rodgers MBBS, MRCGP
Chief Medical Officer

Why take this test?

  • You suspect low T (testosterone) and you want a comprehensive test to make sure
  • You take testosterone replacement and you want to check your hormones and general health
  • You want to check your balance of male and female hormones whilst taking TRT
  • You recognise there can be side effects with TRT and you want to minimise your risks

Laboratories you can trust

Trusted by the NHS and private clinics alike, you can be sure of the highest testing standards from our UKAS accredited partner laboratories.

Dr Sam Rodgers - Chief Medical Officer

Expert advice with your results

Get trusted advice and comments from one of our doctors based on your results.

Your personal health centre

Your personal health centre

Discover the easy way to track your health through our online portal, View your results with doctor's advice, monitor any changes over time and see the improvements you can make to your health with simple lifestyle changes.

Questions? Let us help
Questions? Let us help

Questions? Let us help

Our customer care team is on-hand to help you find the test that's right for you. Get in touch via phone, live chat or email.


Our tests are not a substitute for seeing your doctor, especially if you are suffering symptoms. Our doctors will interpret your results based on the information you have provided, but will not diagnose, consult or provide any treatment. You will be advised to see your doctor for any necessary follow-up action.