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Day 3 Fertility Blood Test

Day 3 Fertility Blood Test


A test to measure your reproductive hormones on the third day of your period to assess egg production and ovarian reserve, which can affect your fertility.
Blood sample 3 biomarkers included Results estimated in 2 working days from sample receipt at lab
Day 3 Fertility Blood Test
Day 3 Fertility Blood Test
Day 3 Fertility Blood Test
Day 3 Fertility Blood Test
Day 3 Fertility Blood Test

Is it for you?

Do you want to start a family either now or in the future and are wondering about your fertility? Or are you actively trying for a baby without success? Perhaps you are just curious about your fertility and want a fertility MOT? If so, this day three fertility test will tell you whether there is a hormonal reason that could affect your chances of becoming pregnant.

What's included?

Biomarker profile

  • Hormones

Hormones (3 Biomarkers)

Special instructions

Take this test between days 2 and 5 of your period. It can be taken at any time if you are not having periods. Do not take biotin supplements for 2 days prior to this test. If you are taking prescribed biotin you should discuss this with your doctor. HRT and the contraceptive pill can affect the results of this test, taking the test during a break from these will give more accurate results. Avoid taking a finger-prick sample from a finger used to handle hormone-gels or hormone-pessaries in the past 4 weeks.

From the expert

This day three fertility test is normally the first test that is performed in any investigation into female infertility. Ideally, the test is taken three days after the start of your period but it can also be taken on days two, four or five of the menstrual cycle. The aim of the test is to check that hormone levels can support the maturation and release of a healthy egg from the ovary. 

Follicle-stimulating hormone (FSH) is released from a gland in the brain (the pituitary gland) to stimulate the ovary to start maturing an egg. Luteinising hormone (LH) is required for the final maturation and release of the egg from a follicle (a fluid-filled sac containing the egg), while the hormone oestradiol both stimulates the growth of the follicle and prepares the womb lining for pregnancy.

The levels of hormones in the body on day three can indicate whether hormonal problems are likely to affect ovulation and conception and whether a condition like polycystic ovaries may be affecting fertility.
Please note that any hormones you are taking for contraception or hormone replacement therapy (HRT) will influence your results. If you are looking for a true picture of your fertility status then please take this test at least 12 weeks after stopping any hormone replacement or contraceptive pill, implant or hormone IUD. It is recommended to wait until your periods have re-established a regular cycle (for you) before taking this test.

Dr Sam Rodgers MBBS, MRCGP
Chief Medical Officer

Why take this test?

  • You are planning to have a baby and you want to check your hormone status
  • You are struggling to conceive and you want to check your fertility
  • You want a general check of your reproductive hormones
  • You want to know your hormone status so that you can plan ahead for starting a family

Laboratories you can trust

Trusted by the NHS and private clinics alike, you can be sure of the highest testing standards from our UKAS accredited partner laboratories.

Dr Sam Rodgers - Chief Medical Officer

Expert advice with your results

Get trusted advice and comments from one of our doctors based on your results.

Your personal health centre

Your personal health centre

Discover the easy way to track your health through our online portal, View your results with doctor's advice, monitor any changes over time and see the improvements you can make to your health with simple lifestyle changes.

Questions? Let us help
Questions? Let us help

Questions? Let us help

Our customer care team is on-hand to help you find the test that's right for you. Get in touch via phone, live chat or email.


Our tests are not a substitute for seeing your doctor, especially if you are suffering symptoms. Our doctors will interpret your results based on the information you have provided, but will not diagnose, consult or provide any treatment. You will be advised to see your doctor for any necessary follow-up action.