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Stress Cortisol Saliva Tests (4)

Stress Cortisol Saliva Tests (4)


Our most popular cortisol saliva test helps you understand your stress levels and adrenal function over the course of a day.
Saliva sample 4 biomarkers included Results estimated in 3 working days from sample receipt at lab
Stress Cortisol Saliva Tests (4)
Stress Cortisol Saliva Tests (4)
Stress Cortisol Saliva Tests (4)

Is it for you?

Do you worry that your job or your day-to-day life is too stressful for good health? Do you think that chronic stress might be affecting your sleep, your food choices - even your energy levels and mood? Do you find it difficult to "switch off" at the end of the day? If so, find out whether your cortisol levels could be affecting your wellbeing with this simple saliva cortisol test you can do at home.

What's included?

Biomarker profile

  • Adrenal Hormones

Adrenal Hormones (4 Biomarkers)

Special instructions

Do not eat or drink for 30 minutes before your test. Do not brush your teeth for 30 minutes before your test. Your result could be affected by corticosteroid medication (e.g. prednisolone). Ask your doctor whether you should stop before testing. HRT and the contraceptive pill can affect the results of this test, taking the test during a break from these will give more accurate results. Do not take biotin supplements for 2 days prior to this test. If you are taking prescribed biotin you should discuss this with your doctor.

From the expert

Cortisol is a steroid hormone, produced by the adrenal glands. It is vital for survival given its role in various functions such as immunity, regulating blood pressure and releasing insulin for blood sugar maintenance. Cortisol has been termed the 'stress' hormone because it is secreted in higher quantities during the body's 'fight or flight' response to stress, and is responsible for several stress-related changes in the body.

Cortisol levels naturally fluctuate throughout the day but sometimes your levels might higher or lower than normal causing symptoms such as difficulty waking in the morning or going to sleep at night and craving sugary snacks especially late in the day. Cortisol levels should be highest just after waking in the morning and lowest at bedtime. This test establishes the levels of cortisol in the saliva at 4 different points in the day. Samples are taken after waking, 12 noon, 4pm and before going to sleep. 

This test can tell you if you are experiencing excessive stress during the day and whether you need to be adjusting your lifestyle to introduce more relaxation and modify other lifestyle factors such as your sleep and your diet. This test can help identify Cushing's syndrome, which is caused by too much cortisol, or Addison's disease, which is caused by damage to the adrenal glands and is associated with too little cortisol. It is also used by some practitioners in the diagnosis of adrenal fatigue, a condition where elevated levels of stress are thought to impair adrenal function.

Please note that the lower detection limit for this test is 1.5 nmol/L.

Dr Sam Rodgers MBBS, MRCGP
Chief Medical Officer

Why take this test?

  • You want to check your that your stress levels aren't too high
  • You crave sugar and carbohydrates late in the day
  • You find it difficult to wake in the morning and to get to sleep at night
  • You feel constantly tired and run down and wonder whether high cortisol is to blame

Laboratories you can trust

Trusted by the NHS and private clinics alike, you can be sure of the highest testing standards from our UKAS accredited partner laboratories.

Dr Sam Rodgers - Chief Medical Officer

Expert advice with your results

Get trusted advice and comments from one of our doctors based on your results.

Your personal health centre

Your personal health centre

Discover the easy way to track your health through our online portal, View your results with doctor's advice, monitor any changes over time and see the improvements you can make to your health with simple lifestyle changes.

Questions? Let us help
Questions? Let us help

Questions? Let us help

Our customer care team is on-hand to help you find the test that's right for you. Get in touch via phone, live chat or email.


Our tests are not a substitute for seeing your doctor, especially if you are suffering symptoms. Our doctors will interpret your results based on the information you have provided, but will not diagnose, consult or provide any treatment. You will be advised to see your doctor for any necessary follow-up action.